Regulatory

Part:BBa_J48100:Design

Designed by: Laina Magaya   Group: iGEM06_PrairieView   (2006-10-23)


Promoter/Met32p


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3160
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4785
    Illegal BamHI site found at 3099
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2934
    Illegal AgeI site found at 3290
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1004
    Illegal SapI site found at 2916


Design Notes

Found on plasmid pUC57 with a unique BamHI and EcoRV site at 5'end and 3'end.


Source

Saccharomyces cerevisiae

References